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M9480823.TXT
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1994-09-05
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Document 0823
DOCN M9480823
TI Rapid identification of Pneumocystis carinii isolates by random
amplification polymorphic DNA fingerprinting.
DT 9410
AU Chary-Reddy S; Graves DC; Univ. of Okla. Hlth. Sci. Ctr., Oklahoma City.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:622 (abstract no. V-27). Unique
Identifier : AIDSLINE ASM94/94313122
AB Pneumocystis carinii is an opportunistic pathogen that causes pneumonia
in immunocompromised hosts including humans. It is the leading cause of
morbidity and mortality in patients with AIDS. To understand more
clearly the transmission of this organism, it is important to develop
methods that can distinguish P. carinii isolates. We utilized a randomly
designed primer P14 (GCA CAC TCT C) and the random amplified polymorphic
DNA (RAPD) assay (Williams et al. 1990. Nucleic acids Res. 18: 6531) to
amplify genomic DNA segments of P. carinii obtained from rat, mouse,
horse, ferret and human. The amplified products were detected by gel
electrophoresis and Southern hybridization using a 32P labelled
repetitive DNA sequence from rat P. carinii. Genomic DNA from P. carinii
isolates of different species gave distinct RAPD profiles, suggesting
that these immunocompromised hosts were infected by genetically distinct
P. carinii strains. Genomic DNA of P. carinii obtained from two
different strains of rats (Sprague-Dawley from Sasco, Inc. and Wistar
from Hilltop laboratories) and Sprague-Dawley rats from different
vendors produced different band patterns. Furthermore, we were able to
demonstrate two different genetic variants of P. carinii in Wistar rats
suggesting a coinfection. In preliminary studies using DNA obtained from
two different human P. carinii isolates, identical RAPD profiles were
observed. Thus, it appears that the RAPD assay using primer P14 is an
attractive method for identifying specific strains of P. carinii and is
suitable for large scale epidemiological studies.
DE Animal AIDS-Related Opportunistic Infections/*DIAGNOSIS Base Sequence
Blotting, Southern/METHODS Comparative Study DNA Primers Ferrets
Gene Amplification/*METHODS Horses Human Mice Molecular Sequence
Data Pneumocystis carinii/GENETICS/*ISOLATION & PURIF Pneumocystis
carinii Infections/*DIAGNOSIS Polymorphism (Genetics) Random
Allocation Rats Rats, Sprague-Dawley Rats, Wistar Repetitive
Sequences, Nucleic Acid Species Specificity Variation (Genetics)
MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).